The coins will begin entering general circulation in the next few weeks, but fans can get their hands on the 50p pieces as they begin to fill the tills at the Royal Mint Experience in Llantrisant, South Wales.
The Royal Mint is also selling “mint condition” editions of the coins which can be purchased for £10 each.
Nicola Howell, director of consumer coin at the Royal Mint, said: “Following the huge popularity of the coins featuring the much-loved bear last year, we thought it was only right that Paddington continued his adventures around London on UK coinage.
“Paddington Bear is a massive part of British popular culture and is a favourite amongst fans of all ages, who we’re sure will be looking out for him in their change, the first of which will go into tills in the Royal Mint Experience.”
Famous for his love of marmalade sandwiches, Paddington first appeared in a book published by then-Blue Peter cameraman Michael Bond, who found a bear alone on a shelf in Selfridges on Christmas Eve in 1956.
“I wrote some stories about the bear, more for fun than with the idea of having them published. After ten days I found that I had a book on my hands.”